ADVANTAGES OF TWO-PHOTON MICROSCOPY UTILIZING FEMTOSECOND FIBER LASERS

Microscope

Traditional microscopy is the technical field of using microscopes to view objects and areas (mostly cells) that are not within the resolution range of the naked eye. Compound microscope designs feature objectives and condensers, but also consists of very simple, single-lens instruments that are often hand-held, such as a photography loupe or common magnifying glass.

Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy). This technique, though useful, has a few drawbacks: it only allows for scanning the surface of tissues, offers relatively low resolution and contrast, and is one-dimensional.

Confocal Microscopy Diagram